Le Moyne masterfully captures the subject in his watercolor and gouache piece "Grapevine", c. 1580. "Grapevine" began as part of a series of botanicals presumably created for a wealthy English patron, though Le Moyne also painted similar pieces, including "Grape-vine" in the Victoria and Albert Museum. By painting reflected lights and dappled shadows from life, Le Moyne creates convincingly round grapes. In comparison, the curved leaves lack the impressive three-dimensionality of the globular grapevine. Twined vines at the top produce interesting negative space, though the narrow path between the leaves and border causes tension. Though it would be another century before Italian painters popularized the technique of chiaroscuro, Le Moyne's juxtaposition of shadowed grapes against a pale vellum background creates the same dramatic effect.